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The 2D image of rat motor cortex (A) with the highlighted protoplasmic astrocyte and the vessel (yellow circle), impregnated neurons (red circle), and neurons stained with cresyl violet (green circle). Ob. 100x, oc. 20x; (B) corresponding 3D surface for the 2D histological image of the same histological section.

Journal: Frontiers in Neuroanatomy

Article Title: A combined use of silver pretreatment and impregnation with consequent Nissl staining for cortex and striatum architectonics study

doi: 10.3389/fnana.2022.940993

Figure Lengend Snippet: The 2D image of rat motor cortex (A) with the highlighted protoplasmic astrocyte and the vessel (yellow circle), impregnated neurons (red circle), and neurons stained with cresyl violet (green circle). Ob. 100x, oc. 20x; (B) corresponding 3D surface for the 2D histological image of the same histological section.

Article Snippet: The transfer of the 2D histological images to 3D surface format (see ) with MATLAB allows seeing all the structures in the 3D landscape that can be further used for stand-alone quantitative analysis with AI technologies.

Techniques: Staining

The 2D image of rat motor cortex (A). The protoplasmic astrocytes (yellow circle) and impregnated neurons (red circle) are in focus, and the neurons stained with cresyl violet are sharp. Ob. 40x, oc. 20x; (B) corresponding 3D surface for the 2D histological image of the same histological section.

Journal: Frontiers in Neuroanatomy

Article Title: A combined use of silver pretreatment and impregnation with consequent Nissl staining for cortex and striatum architectonics study

doi: 10.3389/fnana.2022.940993

Figure Lengend Snippet: The 2D image of rat motor cortex (A). The protoplasmic astrocytes (yellow circle) and impregnated neurons (red circle) are in focus, and the neurons stained with cresyl violet are sharp. Ob. 40x, oc. 20x; (B) corresponding 3D surface for the 2D histological image of the same histological section.

Article Snippet: The transfer of the 2D histological images to 3D surface format (see ) with MATLAB allows seeing all the structures in the 3D landscape that can be further used for stand-alone quantitative analysis with AI technologies.

Techniques: Staining